Coding

Part:BBa_K2091001:Design

Designed by: Ben Vaknin   Group: iGEM16_BGU_ISRAEL   (2016-09-18)


Protocatechuate 3,4-dioxygenase Beta Subunit


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 317
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 244
    Illegal NgoMIV site found at 313
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Designed using Benchling to meet registry standards.
The sequence was amplified from the P. putida KT2440 strain and cloned into the pSB1C3 vector using EcoRI and PstI.

Source

Genomic DNA of Pseudomonas putida strain KT2440.

References

1. Frazee, R. W., Livingston, D. M., LaPorte, D. C., & Lipscomb, J. D. (1993). Cloning, sequencing, and expression of the Pseudomonas putida protocatechuate 3, 4-dioxygenase genes. Journal of bacteriology, 175(19), 6194-6202.‏
2. Bull, C., & Ballou, D. P. (1981). Purification and properties of protocatechuate 3, 4-dioxygenase from Pseudomonas putida. A new iron to subunit stoichiometry. Journal of Biological Chemistry, 256(24), 12673-12680.‏